Rapid and sensitive detection of Acinetobacter baumannii using loop-mediated isothermal amplification.
نویسندگان
چکیده
Here we report the design and evaluation of a loop-mediated isothermal amplification (LAMP) assay for detecting Acinetobacter baumannii DNA based on the 16S-23S rRNA intergenic spacer (ITS) sequence. The results showed that target DNA was amplified and visualized within 30min and with a detection limit 100-fold greater than PCR.
منابع مشابه
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ورودعنوان ژورنال:
- Journal of microbiological methods
دوره 92 2 شماره
صفحات -
تاریخ انتشار 2013